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. 2009 Jun 3;96(11):4598–4609. doi: 10.1016/j.bpj.2009.01.063

Figure 1.

Figure 1

(Top) Schematic of the custom-built monolayer trough. Internal dimensions: 150 × 50 × 2 mm. The central hole has a diameter of 25 mm and is elevated 2 mm over the trough bottom. The observation window, a coverglass No. 00, was glued to a stainless-steel ring that fits exactly into the central hole of the trough. The design allowed extensive cleaning of the coverglass, which was needed to keep the 100 μm aqueous subphase stable above the observation window. (Bottom) Schematic of the in-house-built fluorescence microscopy/spectroscopy setup. L1–L7 are simple achromatic lenses. A flip mirror makes it possible to guide the fluorescence signal either to an EMCCD camera or through a pinhole to the APD. The setup is shown in the WFM configuration. Removal of lens L3 and flipping of the mirror transforms the setup to an FCS configuration.