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. Author manuscript; available in PMC: 2009 Jul 16.
Published in final edited form as: Nat Cell Biol. 2008 Jul 6;10(8):935–945. doi: 10.1038/ncb1753

Figure 1. dRagA and dRagC are novel activators of TORC1 in Drosophila S2 cells.

Figure 1

(a) Knockdown of dRagA and dRagC decreased dS6K phosphorylation (T398). Drosophila S2 cells treated with or without each indicated RNAi were amino acids (AA) starved for 1 h followed by AA stimulation for 30 min. Phosphorylation and protein levels of dS6K were determined by immunoblotting with indicated antibodies.

(b) dRagA and dRagC are not required for dAkt phosphorylation. Drosophila S2 cells treated with or without each indicated RNAi were AA starved for 1 h and stimulated with AA for 30 min. Phosphorylation and protein levels of dAkt were determined by immunoblotting with appropriate antibodies as indicated. NC: negative control RNAi.

(c) dRagA and dRagC function parallel to TSC2 and PTEN. dRagA or/and dRagC RNAi was added to S2 cells in combination with dTSC2 or dPTEN RNAi as indicated. dTSC2 or dPTEN RNAi treatment increased pdS6K (T398) and this increase was compromised by dRagA or/and dRagC RNAi.