Figure 1. PMTs are not differentially expressed and the loss of one pmt gene is not compensated by overexpression of another PMT gene.

The wild-type (JEC21), Δpmt1 and Δpmt4 mutant strains were incubated to mid-logarithmic phase in YPD medium and total RNA was isolated. Quantitative real-time PCR was performed on the corresponding cDNA samples to assess relative PMT1, PMT2, and PMT4 transcript abundance compared to the wild-type strain±one standard deviation. The expression values in all three strains are normalized to the PMT2 expression.