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. Author manuscript; available in PMC: 2010 Jun 15.
Published in final edited form as: Cancer Res. 2009 May 26;69(12):5023–5029. doi: 10.1158/0008-5472.CAN-08-3731

Figure 3. The caspase-8 “RXDLL motif” is essential both for association with Src and for EGF-induced cell motility.

Figure 3

A) Anti-HA immunoblot of anti-c-Src immunoprecipitates from 293T cells transfected with c-Src and either empty vector (E.V. lane 1) or expression vectors for caspase-8-HA (W.T. lane 2), caspase-8R71A-HA (lane 3), caspase-8D73A-HA (lane 4) or caspase-8L74A-HA (lane 5). Anti-HA and anti-c-Src immunoblots of the total cell lysates (TCL) used for immunoprecipitations are shown in the lower panels. B) Anti-endogenous Src immunoblot of anti-HA immunoprecipitates from NB7 cells stably infected with either empty vector (E.V. lane 1) or expression vectors for caspase-8-HA (W.T. lane 2), caspase-8R71A-HA (lane 3), caspase-8D73A-HA (lane 4) or caspase-8L74A-HA (lane 5). Anti-caspase-8 and anti-c-Src immunoblots of the total cell lysates (TCL) used for immunoprecipitations are shown in the lower panels. C) Migration assay of the cells used in Fig. 2C and Fig 3B above was carried out as described. Monolayers were wounded, the cells treated with serum-free media supplemented with 100 ng/mL EGF and allowed to migrate for 24 and 48 h. D) Representative images (48 h) of the cell migration assay described in Fig. 3C.