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. Author manuscript; available in PMC: 2010 Jun 15.
Published in final edited form as: Cancer Res. 2009 May 26;69(12):5023–5029. doi: 10.1158/0008-5472.CAN-08-3731

Figure 4. Caspase-8 appears to modulate EGF signaling via Src.

Figure 4

A) Anti-Src immunoblot of anti-HA immunoprecipitates from NB7+Casp8C360A-HA cells serum-starved for 24 h and then treated with either vehicle or EGF (100 ng/mL) for 3 min. Anti-Src and anti-Caspase-8 immunoblots of the total cell lysates (TCL) used for immunoprecipitations are shown in the lower panels. B) Anti-P-Y416Src immunoblot of anti-HA immunoprecipitates from NB7+Casp8C360A-HA cells serum-starved for 24 h and then treated with either vehicle or EGF (100 ng/mL) for 3 min. Anti-Src and anti-Caspase-8 immunoblots of the total cell lysates (TCL) used for immunoprecipitations are shown in the lower panels. C) Anti-P-Y416Src immunoblot of anti-Src immunoprecipitates from NB7+E.V. or NB7+Casp8C360A-HA cells serum-starved for 24 h and then treated with EGF (100 ng/mL) for 3 min. Anti-Src and anti-Caspase-8 immunoblots of the total cell lysates (TCL) used for immunoprecipitations are shown in the lower panels. D) Immunoblot analysis of vehicle (lanes 1 and 3) or EGF (100 ng/mL, 3 min)-induced EGFR activation in NB7 cells lacking caspase-8 (lanes 1 and 2) or same re-constituted with wild type protein (lanes 3 and 4) is shown.