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. 2009 May 18;185(4):613–627. doi: 10.1083/jcb.200903034

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© 2009 Szakmary et al.

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Figure 7. — The N-terminal region of Yb is required for HH expression in cap cells. (A and B) DAPI images of Yb⁷²/Yb⁷² ovarioles carrying a transgene with an N-terminal deletion (pAS5 in A and pAS7 in B). Underproliferation of follicle cells (FC) is evident by the presence of fused egg chambers, each of which contains multiple germline cysts. The germline development is not obviously affected, as indicated by the presence of groups of 16-nurse cell (NC)/oocyte clusters, with the ploidy of each group increasing in a gradient fashion from apical to basal region of the ovariole. (C–E) The expression of the hh-lacZ reporter gene in ovarioles that are wild-type (C) or Yb⁷²/Yb⁷² carrying the pAS5 (D) or pAS7 (E) transgene is shown. In the wild-type ovariole, both cap cells (CC) and TF cells show robust hh expression. However, in the mutant ovarioles, hh expression is conspicuously missing from cap cells, even though its expression in TF cells is normal. This TF-only expression of hh is similar to that of Yb⁷²/Yb⁷² ovarioles (King et al., 2001), indicating that the N-terminal–deleted Yb transgenes do not restore the expression of hh in cap cells. Bars, 20 µm.