Figure 9.

C-terminal region is required for Yb localization. This figure shows the localization of mutant Yb proteins encoded by five Yb mutant alleles, among which Yb⁷² is a premature mutation introducing a stop codon at amino acid residue 134 (King et al., 2001) and Yb¹, Yb⁴, and Yb⁶ are missense mutations that are genetically null (King and Lin, 1999), whereas the pAS7 transgene encodes the C-terminal half of the Yb protein (Fig. 5). (A–E) Wild-type (A) and Yb mutant (B–E) germaria stained for Yb protein (green) and for 1B1 (red) to outline the somatic cells and to label the spectrosome. Yb¹, Yb⁴, and Yb⁶ mutant proteins are not localized to the Yb body. (F) The localization of the pAS7 mutant Yb protein with the N-terminal half deleted, as detected by anti-His antibody (red) to recognize the Yb body in the wild-type germarium, is shown. The DAPI staining (green) labels the nuclei in the germarium. (G–K) Wild-type (G) and the Yb mutant (H–K) testicular apex stained for Yb protein (green) and for 1B1 (red) to outline the somatic cells and to label the spectrosome. Yb¹, Yb⁴, and Yb⁶ mutant proteins are not localized to the Yb body in the testis, either. F, fusome; S, spectrosome; YB, Yb body. Bar: (A–F) 10 µm; (G–K) 20 µm.