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. 2009 May 18;185(4):657–671. doi: 10.1083/jcb.200811159

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© 2009 Winderlich et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 8. — Vessel enlargement induced by anti–VE-PTP antibodies requires Tie-2. (A) Allantois explants from E8.5 Tie-2 +/+, Tie-2 +/−, or Tie-2 −/− embryos (as indicated) were cultured on gelatin-coated ultrathin glass slides in the presence of polyclonal antibodies against VE-PTP for 22 h. (B) Allantois explants from E8.5 embryos were cultured in medium (untreated) or in the presence of 600 ng/ml Ang1. Subsequently, endothelium was visualized in all samples in A and B by indirect immunofluorescence staining for VE-cadherin. Bars, 50 µm.