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. 2009 Jun 1;185(5):841–858. doi: 10.1083/jcb.200902128

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© 2009 Klebig et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — Loss of Bub1 kinase activity, conserved domain I, or kinetochore binding differentially affect the recruitment of CENP-F, MCAK, and Sgo1 to kinetochores. (A, C, and E) Immunofluorescence images of mitotic control or Bub1 RNAi–treated Bub1 mutant cell lines stained with CENP-F (A), MCAK (C), Sgo1 (E, green), and CREST antisera (red, kinetochores). Bar, 10 µm. (B, D, and F) Quantification of CENP-F (B), MCAK (D), and Sgo1 (F) levels on kinetochores in indicated cell lines relative to Bub1-wt. Insets show a higher magnification view of a single kinetochore. Errors bars represent standard deviation.