Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Jun 1;185(5):797–810. doi: 10.1083/jcb.200810084

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 Jiang et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 3. — Induction of APBs is associated with p53/p21-mediated senescence. (A) SA-β-gal staining of C2, C7, and C8 cells treated with 4OHT or ethanol vehicle for 4 d. SA-β-gal expression was found in 4OHT-treated C7 and C8 cells. (B) TRF1 and DAPI staining of C8 cells treated with 4OHT for 3 d. APBs were found in phenotypically senescent cells. (C) Triple immunostaining of TRF1, BrdU, and p21 in C7 and C8 cells after treatment with 4OHT for 4 d, and with BrdU for 24 h before the end of 4OHT treatment. APBs (visualized here as large TRF1 foci) were found mainly in cells staining positive for p21 and negative for BrdU. Bars: (A) 100 µm; (B and C) 20 µm.