Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Jun 1;185(5):797–810. doi: 10.1083/jcb.200810084

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 Jiang et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 6. — Presence of p21 and its binding partner, PCNA, in APBs. (A) Double immunostaining of TRF1 and p21 in C7 cells treated with 4OHT for 1 d, C8 cells treated with 4OHT for 4 d, and IIICF-T/B3 cells treated with a combination of SV40T and control siRNAs for 4 d. p21 was detected inside APBs. (B) Triple immunostaining of TRF1, p21, and PCNA in IIICF-T/B3 cells treated with a combination of SV40T and control siRNAs for 3 d. p21 and PCNA were colocalized in APBs. (C) The effectiveness of PCNA siRNAs (PCNA-1 and PCNA-6) was demonstrated by Western blots. (D) Triple staining of TRF1, SV40T, and PCNA in IIICF-T/B3 cells treated with combination of SV40T and PCNA-6 siRNAs for 4 d. APBs were still detected in cells depleted of both SV40T and PCNA. Bars, 20 µm.

HHS Vulnerability Disclosure