Figure 5.

hSpindly is dispensable for removal of MAD2 and ZW10 from KTs. HeLa S3 cells were treated with GL2 or hSpindly siRNAs for 48 h and stained with anti-hSpindly (red) and anti-MAD2 antibodies (green) (A) or anti-hSpindly, anti-MAD2 antibodies (red) and CREST serum (green) (B), and DAPI (blue). (C) Cells were treated with GL2 or hSpindly siRNAs for 48 h and stained with anti-ZW10 (green) or anti-MAD2 (red) antibodies, and DAPI (blue). (D) Cells were transfected with either a myc-vector construct or a myc-tagged p50-dynamitin construct for 48 h and treated with MG132 for 1 h. Cells are then stained with anti-myc 9E10 serum, anti-hSpindly (red) and anti–α-tubulin antibodies (green), and DAPI (blue). Bars, 10 µm.