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K-fiber stability after hSpindly depletion. HeLa S3 cells were treated with GL2, hSpindly, or Hec1 siRNAs for 48 h and left at 37°C (A) or placed at 4°C (B) for 20 min, before they were stained with anti–α-tubulin antibody (green), CREST serum (red), and DAPI (blue). Bars, 10 µm.
