Figure 1.

In vivo selection of BAX frameshift mutation in HB5 BAX(+/−) single-cell clone of HCT116 cell line. (Right) The experimental strategy is shown (see text). (Left) Immunostaining with anti-BAX antibodies is shown with a higher magnification of the boundary between stained and unstained regions. The microslide shows the section of the tumor after microdissection of stained (+) and unstained (−) areas. At the top is shown the PCR amplification of the BAX region comprising the (G)₈ tract from the single-cell clone culture before inoculation into nude mice and from microdissected tumor areas.