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. 2009 Jun 15;185(6):1111–1125. doi: 10.1083/jcb.200901029

Figure 4.

Figure 4.

Rok and myosin are necessary for sustaining apical tension but not maintaining AJs. (a) Confocal immunofluorescent localization of DE-cadherin (DE-cad) in single-cell rok2 (Rok null) MARCM clones. (b) Confocal immunofluorescent localization of DE-cadherin in a multiple-cell rok2 MARCM clone. (c–c″) Confocal immunofluorescent localization of DE-cadherin (c and c′) and phospho-MLC (pMLC; c″) in sqhAX3 (MLC null) MARCM clones. (d–d″) Confocal immunofluorescent localization of DE-cadherin (d and d′) and Zip (myosin heavy chain [MHC]; d″) in zip1 (MHC null) MARCM clones. (a–d) Arrows identify clonal cells, and arrowheads identify AJs between clonal cells. Bars, 10 µm.