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. 2009 Jun 15;185(6):1111–1125. doi: 10.1083/jcb.200901029

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© 2009 Warner and Longmore

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — Dia cooperates with Rok to sustain apical tension but does not maintain formed AJs. (a–a″) Confocal immunofluorescent localization of DE-cadherin (DE-cad; a and a′) and Dia (a″) in dia⁵ (Dia hypomorph/LOF) MARCM clones. (b–b″) Confocal immunofluorescent localization of DE-cadherin (b and b′) and Dia (b″) in dia⁵ MARCM clones expressing Dia-RNAi. (c and d) Confocal immunofluorescent localization of DE-cadherin (c–d′) and phalloidin staining (d″) in clones expressing Dia-CA in 38 h APF pupal eyes. Yellow arrows identify clonal cells, whereas blue arrows identify analogous wild-type cells. Yellow arrowheads identify AJs between two clonal cells, whereas blue arrowheads identify AJs between analogous wild-type cells. (e–e″) Confocal immunofluorescent localization of DE-cadherin (e and e′) and Dia (e″) in Rho1⁷² MARCM clones expressing Dia-CA. (f–f″) Confocal immunofluorescent localization of DE-cadherin (f and f′) and Dia (f and f″) in rok² MARCM clones expressing Dia-RNAi. (a, b, e, and f) Arrowheads identify AJs between two clonal cells. Bars, 10 µm.