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. 2009 Jun 15;185(6):1111–1125. doi: 10.1083/jcb.200901029

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© 2009 Warner and Longmore

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 6. — Rho1 does not maintain formed AJs by regulating total cellular DE-cadherin levels. (a) Western blot analysis of 41 h APF pupal eyes. (b) Quantification of DE-cadherin levels from control and Rho1-RNAi tissue across two independent experiments. Data are represented as mean ± SD. (c–c″) Confocal immunofluorescent localization of DE-cadherin (DE-cad; c and c′) and Armadillo (Arm; c and c″) in clones coexpressing Rho1-RNAi and DE-cadherin. (d–d″) Confocal immunofluorescent localization of DE-cadherin (d and d′) and Armadillo (d and d″) in a clone overexpressing DE-cadherin alone. (c and d) Arrowheads identify AJs between two clonal cells. Bars, 10 µm.