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. 2009 Jun 15;185(6):1111–1125. doi: 10.1083/jcb.200901029

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© 2009 Warner and Longmore

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 7. — Rho1 maintains formed AJs by regulating membrane trafficking of DE-cadherin. (a) Confocal immunofluorescent localization of DE-cadherin (DE-cad) in Rho1⁷² MARCM clones. (b) Confocal immunofluorescent localization of DE-cadherin in Rho1⁷² MARCM clones expressing Rab5-DN. (a and b) Arrowheads identify AJs between two clonal cells. (c) Quantification of the ratio of border length positive for DE-cadherin immunofluorescence divided by the total border length between two Rho1⁷² clonal cells or two Rho1⁷² clonal cells expressing Rab5-DN or Rab5-RNAi (AJ index; see Table S4). Data are represented as mean ± SD; *, P = 0.000351 for Rho1 null + Rab5-RNAi; **, P = 0.000066 for Rho1 null + Rab5-DN. (d–d″) Confocal immunofluorescent localization of DE-cadherin (d and d′) and Dlg (d and d″) in Rho1⁷² MARCM clones expressing Rab5-CA. Arrowheads identify AJ disruptions between PECs and cone cells. (e and e′) Confocal immunofluorescent localization of DE-cadherin after DE-cadherin endocytosis assay in Rho1⁷² MARCM clones. Arrowheads identify accumulations of internalized DE-cadherin in Rho1-null clones. (f–f′″) Confocal immunofluorescent localization of DE-cadherin (f and f″) and Rho1 (f and f′″) in the pupal eye expressing Rab5-GFP (f and f′). Arrowheads mark colocalizations between Rab5-GFP, DE-cadherin, and Rho1. This image is 0.75 µm basal compared with other pupal eye images. (g) Confocal immunofluorescent localization of DE-cadherin in Rho1⁷² MARCM clones expressing Rab11-DN. Arrowheads identify AJ disruptions between clonal cells and nonclonal cells. (h) Confocal immunofluorescent localization of DE-cadherin in Rho1⁷² MARCM clones expressing Rab7-DN. Arrowheads identify AJ disruptions between clonal cells. Bars, 10 µm.