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. 2009 Jun 15;185(6):1047–1063. doi: 10.1083/jcb.200811099

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© 2009 Rabl et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 6. — Fcj1 is directly involved in determining the number and the architecture of CJs. (A) Electron micrograph of a mitochondrion in a section of chemically fixed cells overexpressing Fcj1. (B) Distribution of diameters of CJs in wild-type (WT) control strain (W303) containing empty pCM189 plasmid (n = 21) and Fcj1-overexpressing strain (W303) containing pCM189-Fcj1 plasmid (Fcj1↑; n = 40). Cells were grown on nonfermentable, selective minimal media. A histogram of the number of diameters within the indicated ranges was plotted for both strains. (C and D) Fcj1 was down-regulated in a Δfcj1 strain harboring the pCM189-Fcj1 at different times after doxycycline addition. Wild-type control as in B was used. (C) Expression levels of Fcj1 were monitored by Western blot analysis. (D) Phenotypic analysis of down-regulation of Fcj1. The number of CJs and branches per mitochondrial section (m_{0 h} = 26; m_{13.5 h} = 69; m_{23 h} = 49; m_{37.5 h} = 66; m_{47.5 h} = 57) was determined from electron micrographs of chemically fixed whole cells after the indicated time periods of down-regulation (m = number of mitochondrial sections). The number of CJs and cristae branches per mitochondrial section before down-regulation of Fcj1 (0 h) was defined as 100%. The number of rho⁰/rho⁻ cells and of cells containing cristae stacks is related to the number of total cells at each time point.