Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Jun 15;185(6):1047–1063. doi: 10.1083/jcb.200811099

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 Rabl et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 7. — Fcj1 reduces the stability of F₁F_O–ATP synthase oligomers. (A) Protein levels in wild-type (WT), Δfcj1, Δsu e, and Δsu g mitochondria. Aliquots were subjected to SDS-PAGE and decorated with the indicated antibodies. (B) Solubility of F₁F_O–ATP synthase in detergents. Isolated mitochondria of Fcj1-overexpressing (Fcj1↑), wild-type, and Δfcj1 strains were solubilized in digitonin and centrifuged to obtain supernatant (S1) and pellet (P1) fractions. Nonsolubilized material (P1) was treated with Triton X-100 and centrifuged to obtain supernatant (S2) and pellet (P2) fractions. Aliquots were analyzed by Western blotting with the indicated antibodies. (C) BN-PAGE analysis. Wild-type, Δfcj1, and Fcj1-overexpressing mitochondria (400 µg) were solubilized at the indicated ratios of digitonin/protein (Dig/Prot; wt/wt) and subjected to BN-PAGE and to analysis in-gel of F₁F_O–ATPase activity. All lanes were originated from a single gel at identical image settings, but lanes 8 and 9 were cut and pasted in the appropriate order for clarity. (D) SEC. Wild-type, Δfcj1, and Fcj1-overexpressing mitochondria were solubilized at a digitonin/protein ratio of 1 (wt/wt) and fractionated by Superose 6 gel filtration chromatography, and aliquots were analyzed by Western blotting. The oligomeric forms of the F₁F_O–ATP synthase (O, oligomers; T, tetramers; D, dimers; M, monomers) are indicated as size markers.