Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Jun 15;185(6):1029–1045. doi: 10.1083/jcb.200812018

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 Tamura et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 4. — Ups1p and Ups2p affect interactions between the TIM23 translocase and PAM proteins. (A and B) Mitochondria were solubilized with digitonin and subjected to immunoprecipitation with anti-Flag M2-agarose. The mitochondrial lysate (Load; 15%) and bound proteins (Elute; 100%) are shown. Proteins were analyzed by immunoblotting using the indicated antibodies. The asterisk indicates a nonspecific band. Band intensity was normalized relative to that detected in wild-type (WT) mitochondria in B. Values are mean ± SEM (n = 4). (C and D) Immunoprecipitation was performed as described in A using mitochondria expressing Pam16p-Flag. Quantitation of band intensity is shown in D. (E and F) Immunoprecipitation was performed as described in A using mitochondria expressing Tim22p-Flag. (D and F) Values are mean ± SEM (n = 3). co-ip, coimmunoprecipitation.