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. 2009 Jun 15;185(6):983–994. doi: 10.1083/jcb.200904020

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© 2009 Li et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 1. — Generation of Cdc20^AAA allele. (A) A diagram depicting the domain structure of Cdc20 protein. (B) Schematic presentation of the strategy for the generation of Cdc20^AAA. Dashed X's indicate crossing over in homologous recombination. Green arrows indicate PCR genotyping primers. Kn, kanamycin-resistant gene; TK, thymidine kinase. (C) Southern blot analysis of the mutant alleles. (D) PCR genotyping of Cdc20^AAA using primers a and b.