Skip to main content
. 2009 Jun 15;185(6):995–1012. doi: 10.1083/jcb.200903125

Figure 3.

Figure 3.

Dependence of Inn1 localization on the MEN. (A and B) Wild-type (WT; LY1313), cdc5 (LY1357), dbf2 dbf20 (LY1355), and cdc14 (LY1360) cells expressing Inn1-GFP were grown to exponential phase in YM-P–rich medium at 24°C and shifted to 37°C for 3.5 (LY1360) or 2.5 h (the other strains) before imaging (A) and scoring large-budded cells (B) for Inn1-GFP localization. In B, the numbers of cells scored were as follows: 24°C, 62 for wild type and 102–131 for the mutants; 37°C, 78 for wild type and 177–199 for the mutants. The experiments were repeated three times with similar results. Bar, 2 µm.