Table II.
Yeast strains used in this study
| Strain | Genotype | Source |
| YEF473 | a/α his3/his3 leu2/leu2 lys2/lys2 trp1/trp1 ura3/ura3 | Bi and Pringle, 1996 |
| YEF473A | a his3 leu2 lys2 trp1 ura3 | Bi and Pringle, 1996 |
| YEF473B | α his3 leu2 lys2 trp1 ura3 | Bi and Pringle, 1996 |
| Y860 | α his3-11,15 leu2-3,112 trp1-1 ade2-1 can1-100 ura3-1∷URA3:lexAop-ADE2 | C. Boone |
| Y1026 | a his3-11,15 leu2-3,112 trp1-1 ade2-1 can1-100 ura3-1∷URA3:lexAop-lacZ | C. Boone |
| MOY157 | As YEF473B except INN1-GFP:TRP1 HOF1-TAP:His3MX6 cdc15-2 | This studya |
| MOY215 | As YEF473B except INN1-GFP:TRP1 cdc15-2 | This studya |
| MOY609 | As YEF473 except hof1Δ∷TRP1/hof1Δ∷TRP1 INN1/inn1Δ∷kanMX6/ MYO1/myo1Δ∷kanMX6 [pUG36-INN1][pRS315GW-C2-HOF1] | This study |
| MOY630 | As YEF473B except hof1Δ∷TRP1 myo1Δ∷kanMX6 [pUG36-INN1][pRS315GW-C2-HOF1] | Segregant from MOY609 |
| MOY632 | As YEF473B except hof1Δ∷TRP1 innΔ∷kanMX6 [pUG36-INN1][pRS315GW-C2-HOF1] | Segregant from MOY609 |
| MOY634 | As YEF473B except hof1Δ∷TRP1 inn1Δ∷kanMX6 myo1Δ∷kanMX6 [pUG36-INN1] [pRS315GW-C2-HOF1] | Segregant from MOY609 |
| MWY764 | As YEF473A except hof1Δ∷TRP1 iqg1Δ∷His3MX6 [pRS316GW-IQG1] | This study |
| MWY1145 | As YEF473A except hof1Δ∷TRP1 inn1Δ∷kanMX6 [pUG36-INN1] | This study |
| MWY1171 | As YEF473B except cyk3Δ∷kanMX6 inn1Δ∷kanMX6 [pUG36-INN1] | This study |
| RNY2225 | As YEF473A except hof1Δ∷TRP1 chs2Δ∷kanMX6 [pJC328] | This study |
| RNY2393 | As YEF473A except iqg1Δ∷His3MX6 INN1-GFP:kanMX6 [YCp50-IQG1] | This study |
| RNY2395 | As YEF473A except INN1-GFP:kanMX6 [YCp50-IQG1] | This study |
| RNY2494 | As YEF473 except INN1/pINN1-inn1(1–134)-GFP:His3MX6 | This study |
| RNY2498 | As YEF473 except INN1/TRP1:pGAL1-GFP-inn1(1–134):His3MX6 | This study |
| RNY2499 | As YEF473 except INN1/TRP1:pGAL1-GFP-INN1 | This study |
| LY1065 | α hof1Δ∷kanMX6 ade2 ade3 his3 leu2 trp1 ura3 [pTSV30A-HOF1] | This studyb |
| LY1067 | a hof1Δ∷kanMX6 ade2 ade3 leu2 lys2 ura3 [pTSV31A-HOF1] | This studyb |
| LY1302 | As YEF473 except INN1-GFP:kanMX6/INN1-GFP:kanMX6 | This study |
| LY1310 | As YEF473A except inn1Δ∷kanMX6 [pUG36-INN1] | This study |
| LY1313 | As YEF473A except INN1-GFP:kanMX6 | This study |
| LY1314 | As YEF473B except INN1-GFP:kanMX6 | This study |
| LY1321 | As YEF473A except INN1-GFP:kanMX6 cyk3Δ∷His3MX6 | This study |
| LY1324 | As YEF473A except INN1-GFP:kanMX6 | This study |
| LY1325 | As YEF473A except INN1-GFP:kanMX6 cyk3Δ∷His3MX6 hof1Δ∷kanMX6 [pRS316-HOF1] | This study |
| LY1328 | As YEF473A except INN1-GFP:kanMX6 hof1Δ∷kanMX6 [pRS316-HOF1] | This study |
| LY1355 | a dbf2-1 dbf20Δ∷TRP1 INN1-GFP:kanMX6 ade1 leu2 trp1 ura3 | This studyc |
| LY1357 | a cdc5ts∷URA3 INN1-GFP:kanMX6 leu2 trp1 ura3 | This studyc |
| LY1360 | a cdc14 INN1-GFP:kanMX6 can1 his7 leu2 ura3 | This studyc |
| LY1364 | As YEF473A except myo1Δ∷His3MX6 INN1-GFP:kanMX6 [YCp50-MYO1] | This study |
| LY1373 | As YEF473A except inn1Δ∷kanMX6 CHS2-GFP:kanMX6 [pUG36-INN1] | This study |
| YEF1951 | As YEF473A except hof1Δ∷kanMX6 | Vallen et al., 2000 |
| YEF5202 | As YEF473A except inn1Δ∷kanMX6 [pUG34mCherry-INN1-C2] | This study |
| YEF5216 | As YEF473A except inn1Δ∷kanMX6 | This studyd |
| YEF5291 | As YEF473A except inn1Δ∷kanMX6 MYO1-GFP:His3MX6 | This study |
| YEF5293 | As YEF473A except myo1Δ∷His3MX6 INN1-GFP:kanMX6 | This study |
Bolded a′s and α′s indicate mating types of haploid yeast cells. Genes were deleted (the entire coding region in each case) or tagged at their C termini using the PCR method (Baudin et al., 1993). Template plasmids were as described previously by Longtine et al. (1998) except for pFA6a-TAP-His3MX6 (provided by P. Walter, University of California, San Francisco, San Francisco, CA) and pFA6a-link-mCherry-His3MX6 (see Materials and methods). In some cases, genomic DNA from previously transformed strains was used as a template to generate transformation fragments with longer flanking regions. Other steps in strain constructions were conventional genetic crosses and plasmid transformations.
cdc15-2 was derived from strain DLY3034 (provided by D. Lew, Duke University, Durham, NC) and backcrossed more than seven times into the YEF473 background.
Derived from strains CDV38 and CDV39 (provided by C. De Virgilio, University of Fribourg, Fribourg, Switzerland).
Strains J230-2D (provided by L. Johnston, National Institute for Medical Research, London, England, UK), KKY021 (provided by L. Johnston), and 4078-14-3a (provided by L. Hartwell, Fred Hutchinson Cancer Research Center, Seattle, WA) were transformed with a PCR-generated INN1-GFP:kanMX6 cassette.
Generated by growing LY1310 on SC+FOA medium.