Figure 8. Fibrin gels stimulate cell patterning in 2D and 3D.

Characteristic fluorescence image of F-actin structures in hMSCs cultured for 4 days on 16 kPa fibrinogen coated polyacrylamide (A) or 2 mg/ml fibrin (B). C) A bright field image of hMSCs cultured in 3D for 7 days in a 2 mg/ml gel. D) A fluorescently stained junction between two hMSCs in a 3D fibrin gel. For all images F-actin is labeled in red, the nucleus in blue and connexin 43 in green. Scale bar = 100 µm for all images.