Figure 6.

Activation of TRPV2 by temperature jumps. (Bottom) Temperature pulses ranging from 47°C to 58°C. The pulse duration was 100 ms, and the rise time was ∼3 ms. (Top) Currents from an outside-out macropatch from a HEK 293 cell transiently transfected with TRPV2. Holding potential V_h = −60 mV. Significant currents were activated at temperature ≥52°C, and large increases occurred over a temperature change of ∼4°C (from 51°C to 55°C). At the extreme temperature (∼58°C), the current became inactivating, with a peak response similar to the steady-state current at 55°C. The rising phase time constant was ∼34 ms at 55°C.