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. 2009 Jan 7;29(1):280–287. doi: 10.1523/JNEUROSCI.4267-08.2009

Figure 3.

Figure 3.

Effect of combined NPY siRNA and DBH siRNA injection on glucoprivic responses and target gene expression. A shows the effect of DBH siRNA (0.02 nmol) + NPY siRNA (0.02 nmol) (mixed siRNAs) or control ntRNA injected bilaterally into A1/C1, and the effect of the mixed siRNAs injected more dorsally to miss the A1/C1 area (Missed siRNAs), on 4 h saline- or 2DG (200 mg/kg)-induced feeding. Combined siRNAs injected into A1/C1 reduced the 2DG feeding response. **p < 0.001 (vs control). B shows the effect of the same treatments on 2DG-induced hyperglycemia. Glucose levels were measured 15 min before (Pre) and 90 min after (Post) 2DG injection. Blood glucose was significantly increased by 2DG in all groups, but no significant between-group differences were observed. C, Numbers of DBH- and TH-positive cells in A1/C1 by IHC, quantified from rats used in the 2DG tests. DBH siRNA/NPY siRNA mixture injected into A1/C1 significantly inhibited DBH expression, but not TH expression in A1/C1 region. **p < 0.001 (vs control).