Fig. 9.

Cytoskeletal effects of ascorbate on inulin transfer and cellular actin. A: EA.hy926 cells in culture for 6 days were rinsed 3 times in KRH and incubated 37°C with KRH containing 5 mM d-glucose, 0.3 mM DHA, or 2 mM sulfinpyrazone (the latter 2 in the upper well above the filter). After 1 h of incubation, [carboxyl-¹⁴C]inulin (10 μM) was added. After an additional 60 min, the transfer assay was carried out with calculation of inulin permeability across the cells. Results are shown from 5 experiments. *P < 0.05 compared with control. **P < 0.05 compared with treatment without colchicine. B: EA.hy926 cells were cultured on glass slides to near confluence for 4 days and treated for 1 h with or without 300 μM ascorbate as indicated, followed by staining of F-actin with rhodamine-phalloidin as described in materials and methods. Results are shown from 1 experiment typical of 2 such performed.