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Tetracycline-inducible Cre expression in the RPE. (A) Cre expression using the tet-on system. (B) Diagram of transgenic constructs. RNA Pol: RNA polymerase. tetO, tet-operator; PhCMV, immediate early promoter of human cytomegalovirus; rtTA, reverse tet-inducible TransActivator; Dox, doxycycline; PVMD2, promoter of human VMD2 gene; SV40 A(n), simian virus 40 polyadenylation signal; MT-IA(n), mouse metallothionein polyadenylation signal.
