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Expression of Zip3 protein in the mammary gland is eliminated in Zip3-null mice. Representative immunoblot of crude membrane protein extracts (100 μg) isolated from the mammary glands of wild-type (Zip3+/+) and Zip3-null (Zip3−/−) mice (n = 2 mice/genotype). β-actin was immunoblotted to verify equal protein loading.
