Fig. 4.
Antibodies to PTPro increase Palb of isolated glomeruli, an effect blocked by fusion protein. Glomeruli were isolated from normal rats and incubated with a polyclonal antibody to PTPro (PolyAb) with or without preincubation with a glutathione-S-transferase (GST) fusion protein of rat PTPro ECD (Poly+F). Other glomeruli were incubated with 1B4 or 1D1, mAbs to rat PTPro. Each of the three antibodies to PTPro caused a marked increase in Palb, Preincubation with fusion protein prevented the increased Palb caused by the polyclonal antibody to PTPro. Incubation with the irrelevant mAb L11–132 (control) did not affect Palb.