Table 1.

Reagents used in studies of P_alb of rabbit and rat glomeruli

Rabbit Glomeruli
Reagent	Specificity	Concentration, μg/ml	Incubation, min
Antibodies to rabbit PTPro
4C3	Anti-rabbit PTPro ECD mAb, IgG1	5, 10, 25	60, 120
P8E7	Anti-rabbit PTPro ECD mAb, IgG1	25	60, 120
Irrelevant antibodies
BB5	Control mAb, anti-human GBM, laminin binding	25	60, 120
5F7	Control mAb, IgG1 isotype, anti-podocalyxin	55	60, 120
Fusion proteins
Rabbit PTPro FP	GST fusion protein of rabbit PTPro ECD	250	60
Rabbit podocalyxin FP	GST fusion protein of rabbit podocalyxin ECD	250	60

Rat Glomeruli
Reagent	Specificity	Concentration, μg/ml	Incubation, min
Antibodies to rat PTPro
Rat PTPro polyclonal Ab	Polyclonal Ab to rat PTPro ECD	5	60
1B4	Anti-rat PTPro ECD mAb, IgG2a isotype	100	60
1D1	Anti-rat PTPro ECD mAb, IgG2a isotype	100	60
Irrelevant antibodies
Preimmune rabbit serum	Control rabbit serum	0.5–5	10–60
4C3	Anti-rabbit PTPro ECD mAb	5	60, 120
BB5	Control mAb, anti-human GBM, laminin binding	5	60, 120
L11–135	Control mAb, IgG2a isotype, rabbit class II (DQ) proteins	100	60
Fusion protein
Rat PTPro FP	GST fusion protein of rat PTPro ECD	5	60

P_alb, albumin permeability; PTPro, protein tyrosine phosphatase receptor type O; mAb, monoclonal antibody; ECD, extracellular domain; GBM, glomerular basement membrane; FP, fusion protein; GST, glutathione-S-transferase.