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. 2009 Apr 22;297(1):F177–F190. doi: 10.1152/ajprenal.90422.2008

Fig. 2.

Fig. 2.

Representative Western immunoblotting analysis of kidney homogenate using anti-steroidogenic acute regulatory protein (StAR; A–C) and anti-CYP11A1 (D–F) and quantitative real-time PCR measurement of StAR and CYP11A1 (G and H). Kidneys were removed during reperfusion (3 h, 3 days, 7 days) after 24 h of cold preservation (A and D), 60 min of warm ischemia and cold preservation (B and E), or 60 min of warm ischemia without transplantation (C and F); n = 3/group. For StAR, each lane of renal tissue contained 20 μg of cytosolic proteins and, respectively, 2.8m 1.4, and 0.7 μg from testis tissue. For CYP11A1, each lane of renal tissue contained 35 μg of mitochondrial proteins and, respectively, 1.4, 0.7, and 0.35 μg from testis tissue. F: quantitation of StAR mRNA expression in normal kidney (control), kidneys which underwent warm ischemia at body temperature for 60 min (IC 60), kidneys conserved at 4°C for 24 h in University of Wisconsin solution (UW) after 60-min warm ischemia (IC 60 UW), and kidneys conserved at 4°C for 24 in UW (UW). Measurements were performed at both 3 h and 7 days postreperfusion. Testis is used as a positive control for the expression of StAR. G: expression of CYP11A1 in similar tissues and conditions. Values are means ± SE by t-test to control. *P < 0.05, **P < 0.01, and ***P < 0.001.