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. 2009 May 6;297(1):F85–F94. doi: 10.1152/ajprenal.90642.2008

Fig. 5.

Fig. 5.

MCP-1 stimulates podocyte motility as evaluated by a scratch-wound assay. A: in the counting of the number of cells that had repopulated a consistently defined area of the scratch, MCP-1 was seen to significantly stimulate podocyte migration at all time points, resulting in quicker wound closure. A similar effect was seen with TGF-β1 treatment. Both MCP-1- and TGF-β1-induced motility increases were prevented by CCR2 inhibition with RS102895 (n = 4). *P < 0.05 vs. control. †P < 0.05 vs. MCP-1. ‡P < 0.05 vs. TGF-β. B: in a follow-up scratch assay at 42 h, the TGF-β-induced motility gain (gray vs. white bar) was nullified by neutralizing MCP-1 with a specific antibody (α-MCP-1 Ab; red vs. gray bar). A species- and isotype-matched irrelevant antibody (Irr Ab) was used as the control (n = 3). *P < 0.05 vs. control+Irr Ab. ‡P < 0.05 vs. TGF-β+Irr Ab.