Fig. 2.
Effects of SPR gene transfection on tetrahydrobiopterin (H4B) and nitric oxide radical (NO•) bioavailability in vitro. A: transfection efficiency demonstrated by green fluorescence due to green fluorescent protein expression. B: quantification of SPR mRNA expression from cells [control (Ctrl) vs. SPR gene transfection] using real-time PCR. C: SPR protein expression after SPR gene transfection. D: effects of SPR gene transfection on SPR activity monitored by HPLC. E: effects of SPR gene transfection on intracellular H4B level. F: effects of SPR gene transfection on NO• production. Data are shown as means ± SE from 4–10 individual experiments. *P < 0.05 compared with Ctrl.