Fig. 2.
Selectivity of Cx43 wild-type (Cx43wt) and Cx40wt junctions is invariant. Shown are the dye-charge selectivity results for Cx43 junctions (Rin43 cells), Cx40 junctions (Rin40 cells), and cytoplasmic bridges (incompletely divided cells). A: plot of permeance rate constants of NBD-M-TMA vs. Alexa 350 for Cx43 junctions (•), Cx40 junctions (□), and cytoplasmic bridges (▴). These parameters were significantly correlated for all 3 groups with correlation coefficients of 0.98 (n = 17) for Cx43 junctions, 0.99 (n = 15) for Cx40 junctions, 0.97 (n = 5) for bridges. B: average charge selectivities (BNBD-M-TMA/BAlexa 350 ratio) for the experiments plotted in A for bridges 0.91 ± 0.07 (n = 5), Rin43 1.22 ± 0.14 (n = 17), and Rin40 10.7 ± 0.5 (n = 15). *Selectivity of Cx40 junctions was significantly different from Cx43 junctions (Student's t-test).