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Electrophoretic mobility of Cx43, but not Cx40, is altered by phorbol 12-myristate 13-acetate (TPA) treatment of Rin43, Rin40, or A7r5 cells. Total protein was isolated from Rin43, Rin40, and A7r5 cells treated for 0, 15, 30, or 60 min with 100 ng/ml TPA at 37°C. Mobility of Cx43 (left) was altered in both Cx43-expressing cell types, consistent with PKC-induced phosphorylation of Cx43. No changes in the mobility of Cx40 were observed (right).
