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ET-1 (A), endothelial nitric oxide synthase (eNOS; B), and serine 1177 phosphorylated eNOS (PeNOS) protein expression (C) in endothelial cells obtained from antecubital veins in young and older men. Values are means ± SE. HUVEC, human umbilical vein endothelial cell. *P < 0.05 vs. young.