Fig. 3.
Immunohistochemical staining of murine distal colon sections. A: although enterocytes are intensely stained by use of an antibody raised against Kv7.1 (arrows), no immunoreactivity is observed in the smooth muscle layers. B: an antibody raised against Kv7.4 stains both the longitudinal (arrowheads) and circular (asterisks) layers of smooth muscle. The longitudinal layer consistently stained more intensely. The epithelium is also immunoreactive for Kv7.4. C: although the epithelium also contains immunoreactivity for Kv7.5, only faint staining of the circular muscle layer is apparent. D: a representative time-matched section incubated in control serum reveals only very faint false-positive staining of the epithelium only.