Fig. 8.
Expression of β-catenin and genes regulated by β-catenin/T cell factor (Tcf) in small intestine. A and B: immunohistochemical localization of β-catenin in sections of small intestine of 14-day-old wild-type and FGFR-3−/− mice, respectively. Nuclear localization of β-catenin (arrow) was observed in wild-type mice but was rare in FGFR-3−/− mice. C: Western blot analysis of β-catenin in lysates of small intestinal segments of C57BL6/j (B6), FGFR-3−/−, and FGFR-3+/+ mice. D: real-time PCR of total RNA isolated from small intestine of 14-day-old FGFR-3−/− and FGFR-3+/+ mice. Levels of β-catenin mRNA were determined by quantitative real-time PCR. Values are means ± SE (n = 5 mice per group). P = not significant. E: cyclin D1 and matrix metalloproteinase-7 (MMP-7) mRNA levels measured by real-time PCR in RNA isolated from 21-day-old FGFR-3−/− and wild-type mice. Values (means ± SE) are expressed relative to 18S rRNA × 10−3 (n = 3–5 mice per group).