Fig. 9.

Signaling via FGFR-3 maintains β-catenin/Tcf activity in Caco-2 cells. A: luciferase activity in cell lysates from Caco-2 cells transiently transfected with pTOPFlash or Renilla and treated daily with FGF9 (50 ng/ml) starting at 24 h after transfection and harvested 48, 72, 96, and 120 h later (0, 1, 2, and 3 days after confluence, respectively). Cells were cotransfected with an FGFR-3 construct bearing the K650E mutation conferring constitutive activation of the receptor, pUC18 empty vector, or a wild-type FGFR-3 construct. B: β-catenin/Tcf activity expressed relative to luciferase activity from pTOPFlash or Renilla in unstimulated Caco-2 cells. Values are means ± SE (n = 3 culture wells per group for each of 3 independent experiments).