Fig. 5.
Unaltered pharmacokinetics of plasma RBP4 in high-fat diet (HFD)-induced obese mice with elevated plasma RBP4 levels. A: body weight. B: plasma RBP4. C: plasma TTR as determined by Western blotting in HFD-induced obese and chow-fed (CHOW) mice after 16 wk of diet. D: CHOW and HFD mice were injected with hRBP4 (100 µg) via tail vein, and then hRBP4 levels in plasma were measured at 15 min and 1, 2, 4, and 24 h. E: 2-parameter exponential curve fits of hRBP4 levels in plasma of individual mice at 1, 2, 4, and 24 h were used to calculate t1/2 of disappearance from plasma after intravenous injection. F: relative levels of RBP4 and TTR present in the RBP4-TTR complex coimmunoprecipitated from plasma of CHOW or HFD mice using anti-RBP4 antibody and detected by Western blotting with anti-RBP4 antibody or anti-TTR antibody. G: RNA was extracted from liver of lean, ob/ob (left), CHOW, and HFD mice (right), and TTR and 18S mRNA were measured by RT-PCR. TTR mRNA is expressed relative to 18S mRNA. All values are means ± SE. For A, B, and F: *P <0.001 vs. CHOW; n = 7 CHOW and 8–9 HFD. For G: *P < 0.05 vs. lean; n = 6–8 in each group.