Figure 1.

(A) The position of mutations on a DsbB topological model (16). The positions of the four essential cysteines and the two missense mutations are indicated by bold and solid circles, respectively. (B) Sequence alignments of DsbB homologues. The source organisms are: Ec, E. coli; Sf, Shigella flexneri; Va, Vibrio alginolyticus; Hi, Hemophilus influenzae; Ea, Enterobacter aminigenus; Bc, Burkholderia cepacia; Pa, Pseudomonas aeruginosa; Rp, Rickettsia prowazekii; Cj, Campylobacter jejuni; and Bs, Bacillus subtilis. Black highlighting, completely conserved amino acid residue; boldface, amino acid residues identical in more than 60% but less than 100% of the sequences compared; ∗, the position of Arg48.