Fig. 3.

Time-dependent and concentration-dependent attachment of fibroblasts to extracellular matrix proteins. A: time dependency of fibroblast attachment. Fibroblasts (10 × 10³ cells) attached to vitronectin (VN; 5 μg/ml), fibronectin (FN; 10 μg/ml), collagen (CL; 10 μg/ml), or fibrinogen (FGN; 10 μg/ml) in 96-well plates for 30 min in attachment assay buffer. After being washed at indicated times, remaining attached cells were counted. Value of cell attachment to fibrinogen at 60 min was 58.2 ± 4.9% (means ± SE; n = 4) of that to vitronectin at same time point. Results are expressed as percentage of maximal attachment to same matrix protein at 60 min. Values are means ± SE for n = 4. B: concentration dependency of fibroblast attachment. Cell attachment was assayed at either 20 min (for vitronectin, collagen, and fibronectin) or 60 min (for fibrinogen). Results are expressed as percentage of attachment to vitronectin at 20 min. Values are means ± SE for n = 4.