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IL-6 overexpression induces Bax phosphorylation in room air and 100% O2. Lung protein homogenates were immunoprecipitated with anti-Bax antibody and assessed for serine phosphorylation with anti-phosphoserine antibody 7F12, which is associated with enhanced phospho-Akt (p-Akt). IL-6 mice exhibited significantly enhanced phosphorylation of Bax and Akt compared with WT mice in room air and in hyperoxia. Each blot is representative of ≥3 similar evaluations.
