Fig. 3.

Loss of CFTR results in a reduction in the protein levels of the histone deacetylase HDAC2 but not HDAC1. The level of HDAC1 and HDAC2 expression in nuclear extract was determined by Western blotting. Nucleolin is used as a loading control. Protein levels were assayed in IB3/S9 and 16HBE cells. A: Western blot showing protein levels of HDAC1 and HDAC2. 16HBE, 9/HTEo, and S9/IB3 samples were run on different gels. B: densitometry analysis of HDAC2 protein levels relative to nucleolin protein level. n = 3. *P < 0.004 for comparisons of CFTR+ with CFTR− cells for each cell pair. C: densitometry analysis of HDAC1 protein levels relative to nucleolin protein level. n = 3.