Fig. 1.

Real-time RT-PCR amplification of Rh B glycoprotein (Rhbg) and Rh C glycoprotein (Rhcg) mRNA. RNA from murine lung was reverse transcribed and then amplified using gene-specific primers and TaqMan probe for Rhbg and for Rhcg. Amplification of both Rhbg and Rhcg was observed. Kidney mRNA was used as a positive control. No Rhbg or Rhcg amplification was seen in the absence of reverse transcription or in the absence of tissue RNA (data not shown), confirming specific expression of lung mRNA.