Fig. 4.

Activation of sACI/II reversibly reorganizes microtubules necessary for intercellular gap formation. PMVECs were infected with an adenovirus expressing sACI/II, and the endogenous microtubule organization was examined using Tubulin Tracker Green Reagent (250 nmol/l). A: forskolin (10 μmol/l) stimulation of sACI/II reorganized endogenous microtubules and resulted in gap formation. Forskolin washout resulted in recovery of peripheral microtubule architecture over a 3-h time course. B: PMVECs were pretreated with paclitaxel (100 nmol/l) for 3 h, and then stimulated with forskolin (10 μmol/l) for 30 min. Paclitaxel stabilized microtubule structures and prevented forskolin from reorganizing microtubules and inducing intercellular gaps. The scale bars represent 25 μm. Arrows denote intercellular gaps.