Figure 4. The effect of 2-butyne-1-ol and 2-butyne-1,4-diol on diazotrophic growth of A. vinelandii expressing MoFe protein variants.
Cells expressing the MoFe protein variants include: α-191Ala, α-70Ala, and α-191Ala/α-70Ala. The indicated strain was cultured on agar plates made with Burk’s media lacking a fixed nitrogen source and including the following additions: no addition; + 2-butyne-1-ol, 10 mM 2-butyne-1-ol; and + 2-butyne-1,4 diol, 10 mM 2-butyne-1,4 diol.