. Author manuscript; available in PMC: 2009 Jul 16.

Published in final edited form as: J Inorg Biochem. 2007 May 29;101(11-12):1642–1648. doi: 10.1016/j.jinorgbio.2007.05.007

Table 1.

Features of MoFe Protein Variants

A. vinelandii Strain	Amino Acid	Phenotype^a	Inhibition of H₂ evolution by N₂ (%)^b
DJ995 (WT)	α-70^Val	Nif+	63
DJ1313	α-70^Gly	Nif−	0
DJ1310	α-70^Ala	Nif+	37
DJ1242	α-191^Ala	Nif+	38
DJ1495	α-70^Ala/α-191^Ala	Nif+	31

Nif+ is defined as able to grow under diazotrophic conditions, while Nif-as not able to grow under diazotrophic conditions.

for purified proteins in a standard H₂ evolution assay under 1 atm of N₂